Susceptibility of non-thymine containing DNA to four bacterial restriction endonucleases
نویسندگان
چکیده
منابع مشابه
Digestion of DNA with restriction endonucleases.
Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. The first method presented in this unit is the cleavage of a single DNA sample with a single restriction endonuclease. A...
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Data on DNA polymorphisms detected by restriction endonucleases are rapidly accumulating. With the aim of analyzing these data, several different measures of nucleon (DNA segment) diversity within and between populations are proposed, and statistical methods for estimating these quantities are developed. These statistical methods are applicable to both nuclear and nonnuclear DNAs. When evolutio...
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A physical map of the adeno-associated virus type 2 genome has been constructed on the basis of the five fragments produced by the restriction endonucleases HindII + III from Hemophilus influenzae. There are three endo R-HindII cleavage sites and one endo R-HindIII site. Evidence has been obtained to support the existence of two nucleotide sequence permutations in adeno-associated virus DNA, th...
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pBR322/Ultraviolet irradiation/Restriction endonuclease/Pyrimidine dimer Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restric tion endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), a...
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A full length, double-stranded ovalbumin complementary DNA (cDNA) was synthesized in vitro and used to assay the action of 35 restriction endonucleases upon this DNA sequence. Some 22 enzymes, Bal I, BamHl, Blu I and its isoschizomerXho I, Ppu 1,Xba I, Tag I, Sal I, Bgl I, Bgl II, Kpn I, Sst II, Hue II, Hpa I, Hap II, Hha I, Hind11 and its isoschizomer HincII, Hind111 and its isoschizomer Hsu I...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)81011-8